How Andor iXon EMCCD Cameras Present Data
- Quantify data in electrons or incident photons
- Convenient estimate of sample signal intensity at the detector
- Real time or post-convert
- Reference between different samples, users and set-ups
- Meaningful signal relating to PALM/STORM localization accuracy
One of the distinctive features of the iXon family is the capability to quantitatively capture and present data in units of electrons or photons, the conversion applied either in real time or as a post-conversion step.
Photons that are incident on pixels of an array detector are captured and converted to electrons. During a given exposure time, the signal in electrons that is collected in each pixel is proportional to the signal intensity. In EMCCDs, the signal in electrons is further multiplied in the EM gain register. The average multiplication factor is selected in software from the RealGain™ scale.
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